Simultaneous detection and identification of four viruses infecting pepino by multiplex RT-PCR
Identifieur interne : 002107 ( Main/Exploration ); précédent : 002106; suivant : 002108Simultaneous detection and identification of four viruses infecting pepino by multiplex RT-PCR
Auteurs : Beibei Ge [République populaire de Chine] ; Qian Li [République populaire de Chine] ; Guojie Liu [République populaire de Chine] ; Meiguang Lu [République populaire de Chine] ; Shifang Li [République populaire de Chine] ; Hongqing Wang [République populaire de Chine]Source :
- Archives of Virology [ 0304-8608 ] ; 2013-06-01.
Abstract
Abstract: Potato virus M (PVM), pepino mosaic virus (PepMV), tomato mosaic virus (ToMV), and potato virus S (PVS) infect pepino and cause serious crop losses. In this study, a multiplex RT-PCR method was developed for simultaneous detection and differentiation of PVS, ToMV, PepMV and PVM. The method was highly reliable and sensitive; validation was accomplished by testing pepino samples collected from different regions of China. In this survey, PVM, ToMV and PVS were detected in 37.0 %, 31.0 % and 5.5 % of samples tested, respectively, confirming the widespread occurrence of these three viruses in China. PepMV was not detected in any of the samples, which indicated that this virus may not be prevalent in China. The results suggest that the new multiplex RT-PCR method has potential to be used routinely for surveys of pepino for virus infection.
Url:
DOI: 10.1007/s00705-013-1604-z
Affiliations:
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In this study, a multiplex RT-PCR method was developed for simultaneous detection and differentiation of PVS, ToMV, PepMV and PVM. The method was highly reliable and sensitive; validation was accomplished by testing pepino samples collected from different regions of China. In this survey, PVM, ToMV and PVS were detected in 37.0 %, 31.0 % and 5.5 % of samples tested, respectively, confirming the widespread occurrence of these three viruses in China. PepMV was not detected in any of the samples, which indicated that this virus may not be prevalent in China. The results suggest that the new multiplex RT-PCR method has potential to be used routinely for surveys of pepino for virus infection.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Ge, Beibei" sort="Ge, Beibei" uniqKey="Ge B" first="Beibei" last="Ge">Beibei Ge</name></noRegion><name sortKey="Ge, Beibei" sort="Ge, Beibei" uniqKey="Ge B" first="Beibei" last="Ge">Beibei Ge</name><name sortKey="Li, Qian" sort="Li, Qian" uniqKey="Li Q" first="Qian" last="Li">Qian Li</name><name sortKey="Li, Qian" sort="Li, Qian" uniqKey="Li Q" first="Qian" last="Li">Qian Li</name><name sortKey="Li, Shifang" sort="Li, Shifang" uniqKey="Li S" first="Shifang" last="Li">Shifang Li</name><name sortKey="Li, Shifang" sort="Li, Shifang" uniqKey="Li S" first="Shifang" last="Li">Shifang Li</name><name sortKey="Li, Shifang" sort="Li, Shifang" uniqKey="Li S" first="Shifang" last="Li">Shifang Li</name><name sortKey="Liu, Guojie" sort="Liu, Guojie" uniqKey="Liu G" first="Guojie" last="Liu">Guojie Liu</name><name sortKey="Lu, Meiguang" sort="Lu, Meiguang" uniqKey="Lu M" first="Meiguang" last="Lu">Meiguang Lu</name><name sortKey="Wang, Hongqing" sort="Wang, Hongqing" uniqKey="Wang H" first="Hongqing" last="Wang">Hongqing Wang</name><name sortKey="Wang, Hongqing" sort="Wang, Hongqing" uniqKey="Wang H" first="Hongqing" last="Wang">Hongqing Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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